mouse anti collagen type i  (Novus Biologicals)

Journal: Future Science OA

Article Title: The potential of plant-derived exosome-like nanovesicles from Centella asiatica leaves (CA-PDENs) for anti-inflammation and prevention of UVB-induced photoaging

doi: 10.1080/20565623.2026.2654777

Figure Lengend Snippet: Results of antioxidant and pro-collagen effects of CA-PDENs. (A) Graphical depiction of DPPH radical scavenging activity of CA-PDENs and vitamin C within the concentration of 6.25-400 µg/mL, data are presented as mean ± standard deviation (n = 3). (B) Fluorescence intensity in UVB-induced 1BR3 cells represents the level of intracellular ROS after CA-PDENs treatment for 24 hours; data are presented as mean ± standard deviation (n = 3). *p < 0.05, **p-value < 0.01 vs. negative control. (C) Confocal microscopy images showing collagen type I expression in 1BR3 cells after 6 hours of CA-PDENs incubation at the concentrations of 40 and 100 µg/mL (200× magnification). Nuclei were stained with DAPI (blue), while collagen type 1 was labeled with Alexa Fluor 647 (red). Merged images show the localization of collagen type 1 expression in the cytoplasmic region surrounding the nuclei. (D) Collagen type I levels in UVB-induced 1BR3 cells followed by CA-PDENs incubation at 40 and 100 µg/mL for 48 hours, data are presented as mean ± standard deviation (n = 3). **p-value < 0.01 vs. control. Abbreviations: CA-PDENs, Centella asiatica -derived exosome-like nanovesicles; DPPH, 2,2-diphenyl-1-picrylhydrazyl; ROS, reactive oxygen species; UVB, ultraviolet B; DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Cells were incubated overnight at 4 °C with a mouse anti-collagen type I primary antibody (1:500 in 1% BSA; Invitrogen, USA), followed by incubation with an Alexa Fluor 647-conjugated goat anti-mouse IgG secondary antibody (1:500 in 1% BSA; Abcam, USA) for 60 minutes in the dark.

Techniques: Activity Assay, Concentration Assay, Standard Deviation, Fluorescence, Negative Control, Confocal Microscopy, Expressing, Incubation, Staining, Labeling, Control, Derivative Assay

Journal: Science Progress

Article Title: A preliminary preclinical assessment of macromolecular crowding in tissue engineering

doi: 10.1177/00368504251406914

Figure Lengend Snippet: SDS-PAGE (A) and densitometry analysis (B) of collagen type I deposition of human BMSCs after 5 days in culture without (−) and with (+) MMC. # indicates significant ( p < 0.05) difference. N = 3.

Article Snippet: At day 5, collagen type I, collagen type III and collagen type IV deposition was quantified using established immunofluorescence protocols., In brief, cell layers were washed, fixed, blocked, incubated with mouse anti-collagen type I (NB600-408, Novus Biologicals, USA), rabbit anti-collagen type III (Ab7778, Abcam, Ireland) or rabbit anti-collagen type V (Ab7046, Abcam, Ireland) primary antibodies, washed, incubated with AlexaFluor ® 488 goat anti-rabbit (A11008, Invitrogen, Ireland) or AlexaFluor ® 488 goat anti-mouse (A10667, Invitrogen, Ireland) secondary antibodies, washed and stained with DAPI (Invitrogen, Ireland).

Techniques: SDS Page

Journal: Science Progress

Article Title: A preliminary preclinical assessment of macromolecular crowding in tissue engineering

doi: 10.1177/00368504251406914

Figure Lengend Snippet: Immunofluorescence (A) and normalised to cell number image intensity analysis (B) of collagen type I, collagen type III and collagen type IV deposition of human BMSCs after 5 days in culture without (−) and with (+) MMC. # indicates significant ( p < 0.05) difference. Scale bar: 100 μm. N = 4.

Article Snippet: At day 5, collagen type I, collagen type III and collagen type IV deposition was quantified using established immunofluorescence protocols., In brief, cell layers were washed, fixed, blocked, incubated with mouse anti-collagen type I (NB600-408, Novus Biologicals, USA), rabbit anti-collagen type III (Ab7778, Abcam, Ireland) or rabbit anti-collagen type V (Ab7046, Abcam, Ireland) primary antibodies, washed, incubated with AlexaFluor ® 488 goat anti-rabbit (A11008, Invitrogen, Ireland) or AlexaFluor ® 488 goat anti-mouse (A10667, Invitrogen, Ireland) secondary antibodies, washed and stained with DAPI (Invitrogen, Ireland).

Techniques: Immunofluorescence